![]() A method for detecting or measuring antigens by using antibodies as reagents is called an immunoassay. Such antibodies can be used as regents for sensitive diagnostic tests (e.g., to detect hepatitis B virus in blood and other tissues, a purpose of the claimed invention). During a hepatitis B infection (or when purified HBsAg is injected experimentally), the body begins to make antibodies that bind tightly and specifically to HBsAg. As its name implies, it is capable of serving as an antigen. For example, on the surface of hepatitis B virus particles there is a large protein called hepatitis B surface antigen (HBsAg). However, it is only after exposure to an antigen that a complicated immune response leads to the production of antibodies against that antigen. The body has the ability to make millions of different antibodies that bind to different antigens. ![]() An antibody has the potential to bind tightly to another molecule, which molecule is called an antigen. ![]() ![]() Antibodies are a class of proteins (immunoglobulins) that help defend the body against invaders such as viruses and bacteria. The claimed invention involves immunoassay methods for the detection of hepatitis B surface antigen by using high-affinity monoclonal antibodies of the IgM isotype. ![]()
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